Leica Sp2

27.01.12 / Leica / Author: admin

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Video: Focal Ca2+ Transient Detection in Smooth Muscle

Transients are common in smooth muscle, yet are difficult to measure accurately because of the problems caused by their stochastic occurrence, over an often wide field of view, in an organ that it prone to contract. To overcome this problem, we've developed a series of imaging protocols and analysis routines to acquire and then analyse, in an automated fashion, the frequency, location and amplitude of such events. While this approach may be applied in other contexts, our own work involves the detection of local purinergic Ca transients allow the optical monitoring of neurotransmitter release in a manner that has many advantages over electrophysiology. Apart from the greatly improved spatial resolution, optical recording has the additional advantage of allowing the recording of transmitter release from many distinguishable sites simultaneously. Furthermore, the optical plane of focus is easier to maintain or correct during long recording series than is the repositioning of an intracellular sharp microelectrode.

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490 pages	Cell imaging techniques, methods and protocols Creator: Douglas J. Taatjes, Brooke T. Mossman | Medical - 2006 The Leica SP2 contained three different lasers (50-mW argon, 1.2-mW HeNe [543 nm ], and 10-mW HeNe [633 nm]) with the multiple wavelengths aligned with a ... Publisher: Humana Pr Inc

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